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Published In
Archives of Rheumatology
Volume 38 · Issue 1 · March 2023
Pages: 119-128
History
Published Online: March 31, 2023
This work is licensed under a Creative Commons Attribution 4.0 International License.
Shanshan Liu*
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
Kai Wang*
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
Ju Li
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
Yan Liu
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
Zhongyuan Zhang
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
Deqian Meng
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
Shanshan Liu*, Kai Wang*, Ju Li, Yan Liu, Zhongyuan Zhang, & Deqian Meng. (2023). MiR-30e-5p deficiency exerts an inhibitory effect on inflammation in rheumatoid arthritis via regulating Atl2 expression. Archives of Rheumatology, 38(1), 119–128. https://doi.org/10.46497/ArchRheumatol.2023.9526
Abstract
Objectives: This study aims to investigate the inflammatory effect of the microRNA (miRNA) miR-30e-5p on rheumatoid arthritis (RA) development in RA mice and fibroblast-like synoviocytes (FLS).
Materials and methods: MiR-30e-5p and atlastin GTPase 2 (Atl2) expression in RA tissues and RA-FLS was evaluated using real-time quantitative polymerase chain reaction. The function of miR-30e-5p in inflammation of RA mice and RA-FLS was analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting. 5-ethynyl-2ˊ-deoxyuridine (EdU) assay was used to detect RA-FLS proliferation. Luciferase reporter assay was to confirm the interaction between miR-30e-5p and Atl2.
Results: MiR-30e-5p expression was upregulated in the tissues from RA mice. Silencing miR-30e-5p alleviated inflammation in RA mice and RA-FLS. MiR-30e-5p negatively modulated Atl2 expression. Atl2 knockdown exerted a proinflammatory effect on RA-FLS. Atl2 knockdown rescued the inhibitory effect of miR-30e-5p knockdown on proliferation and inflammatory response of RA-FLS.
Conclusion: MiR-30e-5p knockdown inhibited the inflammatory response in RA mice and RA-FLS through Atl2.
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Article Info
Published In
Journal
Archives of Rheumatology
Volume / Issue
Volume 38 · Issue 1 · March 2023
Pages
119-128
History
Published Online
March 31, 2023
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Affiliations
1
Shanshan Liu*
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
2
Kai Wang*
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
3
Ju Li
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
4
Yan Liu
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
5
Zhongyuan Zhang
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
6
Deqian Meng
Department of Rheumatology, the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huaian, China
Cite this Article
Shanshan Liu*, Kai Wang*, Ju Li, Yan Liu, Zhongyuan Zhang, & Deqian Meng. (2023). MiR-30e-5p deficiency exerts an inhibitory effect on inflammation in rheumatoid arthritis via regulating Atl2 expression. Archives of Rheumatology, 38(1), 119–128. https://doi.org/10.46497/ArchRheumatol.2023.9526
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